[A+ 실험레포트] Polymerase Chain Reaction (PCR), 원리, 방법, 결과, 고찰

소개글

A+ 받은 실험 레포트입니다.
PCR의 원리, 방법까지 자세하게 적었으며, 영문작성되어있습니다.

목차

1. Subject

2. Object

3. Instruction
I.PCR
1)Definition
2)Components of PCR
3)Steps and principles

II.Gel electrophoresis
1)Types of gel
2)Loading samples and running an agarose gel
3)Visualization

III.PCR product purification (Gel extraction)

4. Materials

5. Methods

6. Results

7. Discussion

본문내용

Subject: Polymerase Chain Reaction (PCR)

Object: Conduct a PCR, electrophoresis and gel extraction and know principles of them

Introduction
I. PCR
1) Definition
Polymerase Chain Reaction, PCR, is a relatively simple and cost-effective way to copy or amplify small segments of DNA or RNA. Using PCR, millions of copies of a section of DNA are made in just a few hours, yielding enough DNA required for analysis. PCR allows clinicians to diagnose and monitor disease using a minimal amount of sample, such as blood or tissue.

2) Components of PCR
- DNA template: the sample DNA that contains the target sequence
- DNA polymerase: a type of enzyme that synthesize new strands of DNA
complementary to the target sequence
- Primers: short pieces of DNA that are made in a laboratory. Primers are necessary because DNA polymerase can’t attach at just any old place and start copying away.

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