HPLC cleaning and generation

소개글

이 ppt는 HPLC 사용에 있어서 중요한 세척과 column 관리 방법에 관한 내용 입니다.

목차

Column?

What causes contaminant in columns?

Washing bonded silica column

Cleaning Protein Residues from Bonded-Silica Reversed-Phase Columns

Special Techniques for Cleaning Bonded-Silica Reversed-Phase Columns

Regeneration for Polymeric Columns

Regeneration of Zirconia-Based HPLC Columns

conclusions

본문내용

Salts, lipids, fatty compounds, humic acids, hydrophobic proteins and other biological compounds are a few of the possible substances that can come in contact with an HPLC column during its use.
Those compounds that have lesser retention, such as salts, will usually be eluted from the column at the void volume.
These undesired interferences might be observed by a detector and appear as chromatographic peaks, blobs, baseline upsets or even negative peaks.
If sample matrix components are retained strongly on the column and if the mobile-phase solvent composition itself never becomes strong enough to elute them, these adsorbed or absorbed compounds will accumulate, usually at the head of the column, after many injections.
Sample compounds that are of intermediate retention can be eluted slowly and appear as wide peaks, baseline disturbances or baseline drift.
Sometimes the sorbed sample components build up to levels high enough that they begin to act as a new stationary phase.

참고 자료

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