Original Article : The Effects of Combined Treatment with an HMG-CoA Reductase Inhibitor and PPARγ Agonist on the Activation of Rat Pancreatic Stellate Cells

저작시기 2012.01 |등록일 2012.09.11 파일확장자어도비 PDF (pdf) | 8페이지 | 가격 4,000원
다운로드
장바구니관심자료
상세신규 배너

* 본 문서는 배포용으로 복사 및 편집이 불가합니다.

서지정보

발행기관 : 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 수록지정보 : Gut and Liver / 6권 / 2호 / 262 ~ 269 페이지
저자명 : ( Beom Jae Lee ) , ( Hong Sik Lee ) , ( Chang Duck Kim ) , ( Sung Woo Jung ) , ( Yeon Seok Seo ) , ( Yong Sik Kim ) , ( Yoon Tae Jeen ) , ( Hoon Jai Chun ) , ( Soon Ho Um ) , ( Sang Woo Lee ) , ( Jai Hyun Choi ) , ( Ho Sang Ryu )

없음

영어 초록

Background/Aims: Hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors (statins) and peroxisome proliferator-activated receptor gamma (PPARγ) ligands can modulate cellular differentiation, proliferation, and apoptosis through various pathways. It has been shown that HMG-CoA reductase inhibitors and PPARγ agonists separately inhibit pancreatic stellate cell (PaSC) activation. We studied the effects of a combination of both types of drugs on activated PaSCs via platelet-derived growth factor (PDGF), which has not previously been reported. The present study was performed to elucidate the underlying mechanisms of these effects by focusing on the impact of the signaling associated with cellcycle progression. Methods: Primary cultures of rat PaSCs were exposed to simvastatin and troglitazone. Proliferation was quantifi ed using the BrdU method, and cell-cycle analysis was performed using a fluorescent activated cell sorter. The protein expression levels of smooth muscle actin (SMA), extracellular signal-regulated kinase (ERK), and a cell cycle machinery protein (p27Kip1) were investigated using Western blot analysis. Results: Simvastatin reversed the effects of PDGF on cell proliferation in a dose-dependent manner. The combination of a low concentration of simvastatin (1 mM) and troglitazone (10 mM) synergistically reversed the effects of PDGF on cell proliferation but had no effect on cell viability. The expression of a-SMA was markedly attenuated by combining the two drugs, which blocked the cell cycle beyond the G0/G1 phase by reducing the levels of phosphorylated ERK and reversed the expression of p27Kip1 interrupted by PDGF. Conclusions: Simvastatin and troglitazone synergistically inhibited cell proliferation in activated PaSCs by blocking the cell cycle beyond the G0/G1 phase. This inhibition was due to the synergistic modulation of the ERK pathway and the cell cycle machinery protein p27Kip1. (Gut Liver 2012;6:262-269)

참고 자료

없음
  • 구매평가(0)
  • 구매문의(0)
      최근 구매한 회원 학교정보 보기
      1. 최근 2주간 다운받은 회원수와 학교정보이며
         구매한 본인의 구매정보도 함께 표시됩니다.
      2. 매시 정각마다 업데이트 됩니다. (02:00 ~ 21:00)
      3. 구매자의 학교정보가 없는 경우 기타로 표시됩니다.
      4. 지식포인트 보유 시 지식포인트가 차감되며
         미보유 시 아이디당 1일 3회만 제공됩니다.

      찾던 자료가 아닌가요?아래 자료들 중 찾던 자료가 있는지 확인해보세요

      상세하단 배너
      우수 콘텐츠 서비스 품질인증 획득
      최근 본 자료더보기
      상세우측 배너
      상세우측 배너
      상세우측 배너
      Original Article : The Effects of Combined Treatment with an HMG-CoA Reductase Inhibitor and PPARγ Agonist on the Activation of Rat Pancreatic Stellate Cells
      페이지
      만족도 조사

      페이지 사용에 불편하신 점이 있으십니까?

      의견 보내기