ㆍ발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)ㆍ수록지정보 : Journal of Microbiology and Biotechnology / 28권 / 3호 ㆍ저자명 : ( Zixing Dong ) , ( Xiaoling Chen ) , ( Ke Cai ) , ( Zhixin Chen ) , ( Hongbin Wang ) , ( Peng Jin ) , ( Xiaoguang Liu ) , ( Kugenthiren Permaul ) , ( Suren Singh ) , ( Zhengxiang Wang )
Owing to its high protein secretion capacity, simple nutritional requirements, and GRAS (generally regarded as safe) status, Bacillus licheniformis is widely used as a host for the industrial production of enzymes, antibiotics, and peptides. However, as compared with its close relative Bacillus subtilis, little is known about the physiology and stress responses of B. licheniformis. To explore its temperature-stress metabolome, B. licheniformis strains ATCC 14580 and B186, with respective optimal growth temperatures of 42℃ and 50℃, were cultured at 42℃, 50℃, and 60℃ and their corresponding metabolic profiles were determined by gas chromatography/mass spectrometry and multivariate statistical analyses. It was found that with increased growth temperatures, the two B. licheniformis strains displayed elevated cellular levels of proline, glutamate, lysine, pentadecanoic acid, hexadecanoic acid, heptadecanoic acid, and octadecanoic acid, and decreased levels of glutamine and octadecenoic acid. Regulation of amino acid and fatty acid metabolism is likely to be associated with the evolution of protective biochemical mechanisms of B. licheniformis. Our results will help to optimize the industrial use of B. licheniformis and other important Bacillus species.