Culture conditions for mycelial growth of Poria cocos
(주)코리아스칼라
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- 2016.05.03
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- 2016.03
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서지정보
ㆍ발행기관 : 한국버섯학회
ㆍ수록지정보 : 한국버섯학회지 / 14권 / 1호
ㆍ저자명 : Woo-Sik Jo, Ju-Ri Park, So-Ra Oh, Min-Gu Kang, Woo-Hyun Kim, Seung-Chun Park
목차
Introduction
Materials and Methods
Fungal isolates
Culture media
Temperature
Effect of pH
Effect of favorable nutrient sources
Results and Discussion
Screening of the suitable culture media
Acknowledgement
Reference
영어 초록
This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and 35oC, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and MgSO4·7H2O and FeSO4·7H2O as mineral salts.
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