비외과적 수정란 이식에 의한 형질전환 소 생산 기술
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국수정란이식학회
ㆍ수록지정보 : Journal of Embryo Transfer / 28권 / 3호
ㆍ저자명 : 엄상준, 양정석, 이수민, 조소영, 허영태, 허영남, 구본철, 정기수, 김광재, 김지태, 김남형, 고대환
ㆍ저자명 : 엄상준, 양정석, 이수민, 조소영, 허영태, 허영남, 구본철, 정기수, 김광재, 김지태, 김남형, 고대환
영어 초록
Recently, the transgenic animal production technique is very important for the production of bio-parmaceutical as animal bio-reactor system. However, the absence of survival evaluation in vitro produced transgenic embryos has been a problem of the low productivity of transgenic animal because of absent of pre-estimate of pregnancy after transgenic embryos transferred into recipient. Therefore, this study is conducted to improve efficiency of transgenic cattle production by improving the non-surgical embryo transfer (ET) method. Transgenic bovine embryos were produced by injection of feline immunodeficiency virus enhanced green fluorescent protein (FIV-EGFP) lentiviral vector into perivitelline space of in vitro matured MІІ stage oocytes, and then in vitro fertilization (IVF) was occured. Normal IVF and EGFP expressing blastocysts were transferred into recipients. Results indicated that 2 expanded blastocysts (34.7%) transferred group showed significantly (P<0.05) higher pregnancy rate than 1 expanded blastocyst (26.8%) transferred group. In case of parity of recipient, ET to heifer (34.9%) showed significantly (P<0.05) higher pregnancy rate than ET to multiparous recipient (21.2%). However, there are no significant differences of pregnancy rate between natural induced estrus and artificial induced estrus groups. Significantly (P<0.05) higher pregnancy rate was obtained from recipient group which have normal corpus luteum with crown group (34.8%) than normal corpus luteum without crown (13.6%). Additionally, treatment of 100 μg Gn-RH injection to recipient group (38.6%) 1 day before ET significantly (P<0.05) increase pregnancy rate than non- Gn-RH injection to recipient group (38.6%). We also transferred 2 EGFP expressing expanded blastocysts to each 19 recipients, 7 recipients were pregnant and finally 5 EGFP transgenic cattle were produced under described ET condition. Therefore, our result suggested that transfer of 2 good-quality expanded blastocysts to 100 μg of Gn-RH injected recipient which have normal corpus luteum with crown is feasible to produce transgenic cattle.참고 자료
없음"Journal of Embryo Transfer"의 다른 논문
- 돼지 단위 발생 난자의 체외 발달에 있어서 피라칸타 추출액의 처리 효과8페이지
- α1,3-Galactosyltransferase 유전자 좌위에서 Membrane Cofactor P..7페이지
- α-1,3-Galactosyltransferase Knock Out(GalT KO) 돼지유래 골수 ..7페이지
- 돼지 자궁내막 세포의 3차원 배양과 Plasminogen Activator 활성화 분석8페이지
- 말의 정소상체 정자의 동결 후 해동 온도 및 Incubation의 효과36페이지
- Monothiolglycerol이 동결 융해 후 미니돼지 정자의 활성산소 억제, 미토콘드리아 활성 ..7페이지
- 돼지의 배란 전 자궁내막 상피세포 내 Plasminogen Activators의 발현7페이지
- Hh-Ag1.5 처리가 돼지 체외수정란의 발육 및 세포사멸에 미치는 영향6페이지
- 혈액 내 코티졸의 패턴 변화가 발정 암캐의 P4 변화와 배란에 미치는 영향5페이지
- 체세포 복제 자견의 성장 및 혈액학적 특성 분석7페이지