내동제의 종류와 농도, PVP 첨가가 돼지 수정란의 Vitrification 동결 융해 시 생존율에 미치는 영향

서지정보

ㆍ발행기관 : 한국수정란이식학회 ㆍ수록지정보 : Journal of Embryo Transfer / 20권 / 2호
ㆍ저자명 : 임정훈, 전연화, 이규승, 김상근

한국어 초록

본 연구는 돼지 수정란의 동결에 있어서 vitrification 동결 융해 후 내동제의 종류완 농도, PVP 및 sucrse와 trehalose의 첨가가 생존율에 미치는 영향을 조사하고자 수행하였다. 1 Vitrification동결에 이용된 각 발생단계의 체외수정란은 1,063개 중 2세포기는 245개, 배반포는 , 초기 배반포는 , 확장 배반포는 221개 , hatching 배반포는 107개 이었다. 상실배, 초기 배반포 및 확장배반포를 EDS와 ET

영어 초록

The present study examines the effects of kinds and concentrations of cryoprotectants, PVP and sucrose and trehalose on the survival rate of vitrified porcine embryos. 1. The developmental stages for the embryos used in vitrification were for 2 cell stage, for the blastocyst, for the early blastocyst from the expanded blastocyst and from hatching blastocyst out of 1,063 embryos. 2 The survival rate of morula, early blastocyst and expanded blastocyst vitrification-thawed with EDT and EGS were , respectively. The expanded blastocyst treated with EDS showed the highest survival rate compared with the other cryoprotectants. 3. The survival rate of early blastocyst, expanded blastocyst and hatching blastocyst vitrification-thawed with EDS diluted in FCS were , respectively. This result were love. than the control of group . 4. The survival rate of embryos vitrified with EDS and EDT supplemented with PVP were , respectively. The survival rate of vitrified embryos cultured for hours were which were significantly lower than that of non-cultured embryos. The survival rate of embryos vitrified with EDS and EDT supplemented between PVP did not have a significant difference. 5. The survival rate of embryos vitrification-thawed with EDS to morula, early blastocyst, expanded blastocyst and hatching blastocyst were to morula, to early blastocyst, to expanded blastocyst and to hatching blastocyst.

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