Relationship between Sloan-Kettering Virus Expression and Granulosa Cells of Atretic Follicles in the Rat Ovary

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 3호
ㆍ저자명 : Hyun Kim, Takashi Matsuwaki, Keitaro Yamanouchi, Masugi Nishihara, Sungwoo Kim, Yeoung-Gyu Ko, Boh-Suk Yang

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Animals Animal Models Extraction of Total RNA Reverse Transcription-Polymerase Chain Reaction (RTPCR) Determination of Apoptotic Cells by TUNEL Method RESULTS Expression of c-Ski Gene in Various Tissues Localization of Ski Protein in the Follicles of Adult Female Rats Ski Protein Expression in Granulosa Cells of Atretic Follicles Localization of Ski Protein in the Follicles of Single Generation in eCG-Primed Immature Hypophysectomized Rats DISCUSSION REFERENCES

영어 초록

Sloan-Kettering virus gene product of a cellular protooncogene c-Ski is an unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. The aim of the present study was to locate Ski protein in rat ovaries in order to predict the possible involvement of Ski in follicular development and atresia. First, expression of c-Ski mRNA in the ovaries of adult female rats was confirmed by RT-PCR. Then, ovaries obtained on the day of estrus were subjected to immunohistochemical analysis for Ski and proliferating cell nuclear antigen (PCNA) in combination with terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). Ski was expressed in granulosa cells that were positive for TUNEL, but negative for PCNA, regardless of the shape and size of follicles. Expression of Ski in TUNEL-positive granulosa cells, but not in PCNA-positive granulosa cells, was also verified in immature hypophysectomized rats having a single generation of developing and atretic follicles by treatment with equine chorionic gonadotropin (eCG). These results indicate that Ski is profoundly expressed in the granulosa cells of atretic follicles, but not in growing follicles, and suggest that Ski plays a role in apoptosis of granulosa cells during follicular atresia.

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