한우 체외수정란 생산을 위한 α-Tocopherol첨가와 동결 효과

서지정보

· 발행기관 : 한국동물번식학회
· 수록지 정보 : Reproductive & developmental biology / 35권 / 3호
· 저자명 : 정진우, 손준규, 최창용, 서상원, 최진석, 조인철, 강태영, 한상현, 강민수, 김덕임, 김현종, 조상래

목차

ABSTRACT 서론 재료 및 방법 난포란의 체외성숙과 수정 수정란의 체외 배양 α-Tocopherol 첨가 배양 초자화 동결 및 융해 수정란의 세포수 조사 통계 분석 결과 및 고찰 α-Tocopherol 첨가에 따른 분할율 α-Tocopherol 첨가에 따른 배반포 발달율 α-Tocopherol 첨가에 따른 총 세포수 α-Tocopherol 첨가에 따른 초자화 동결 · 융해 후 총세포수 적요 인용문헌

영어초록

The aim of this study was to evaluate the effect of α-tocopherol on blastocysts development and subsequent cryosurvival of the vitrification. The α-tocopherol(0, 100, 200, 400 μM) was added in to culture medium for the bovine embryos. The blasocysts from the α-tocopherol and untreated control groups were then frozen-thawed, and their cryosurvival was assessed by in vitro culture for 48 h. There were no differences in the overall cleavage rate(56.14±4.66, 58.18±4.70, 62.97±6.86 and 51.17±7.28) among four treatment groups. However, in blastocyst development and total cell number were significantly higher in α-tocopherol 200 μM(38.60±7.12; 106.33±3.50) to culture medium than other treatment groups(29.30±5.24, 31.60±7.12 and 26.37±4.18; 101.36±5.12, 97.27±2.87, and 91.23±7.52 respectively). Before and after vitrification, the total cell number and blastocyst development of embryo were significantly higher in July to August than September to October. In conclusion, addition of α-tocopherol 200 μM to in vitro bovine embryo culture medium was beneficial for improving embryo quality by decreasing the embryo damage blsstocysts cell number and improving the tolerance of the embryos to cryopreservation.

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