Effect of Epididymal Fluid Fractionated by Chromatography on In Vitro Maturation of Porcine Follicular Oocytes

최초 등록일
2016.04.01
최종 저작일
2010.12
7페이지/파일확장자 어도비 PDF
가격 4,000원 할인쿠폰받기
판매자(주)코리아스칼라
다운로드
장바구니
자격시험 기출문제풀이 - 서비스 오픈 이벤트

* 본 문서는 배포용으로 복사 및 편집이 불가합니다.

서지정보

발행기관 : 한국동물번식학회 수록지정보 : Reproductive & developmental biology / 34권 / 4호
저자명 : Byung Ki Kim, Hye Rim Kim

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Culture Medium Preparation of Epididymal Fluid Chromatography of Porcine Epididymal Fluid RESULTS DISCUSSION REFERENCES

영어 초록

The aim of this study was to investigate what protein(s) of porcine epididymal fluid (pEF) are able to enhance the nuclear maturation of porcine germinal vesicle (GV) oocytes in vitro. Proteins of pEF were fractionated by affinity, ion exchange, and gel filtration chromatography. Porcine cumulus-oocytes complexes (COC) from follicles were cultured in tissue culture medium (TCM 199) containing various fractions obtained by chromatography. Porcine COCs were also cultured in TCM 199 containing various meiosis inhibitors and pEF. After 24 or 48 h culture, oocytes were examined for evidence of GV breakdown, metaphase I, anaphase-telophase I, and metaphase II. When porcine COCs were cultured in the medium with meiosis inhibitor such as, dibutyryl cAMP (dbcAMP) and forskolin (Fo), more than 80% of oocytes were unable to resume meiosis. However, porcine COCs supplemented with pEF were able to overcome the inhibitory effect of dbcAMP and Fo. Maturation rate of oocytes was significantly (p<0.05) increased in the media supplemented with cationic protein(s) during in vitro maturation than in those with anionic protein(s) (44.1% vs 20.0%). When oocytes were cultured in the TCM 199 with fractions obtained by gel filtration, the maturation rate of oocytes was significantly (p<0.05) higher in fraction 11 containing 18 kDa than other fractions. The present study suggests that 1) dbcAMP and Fo prevent the spontaneous maturation of oocyte after isolation from follicles, and that pEF contain a substance(s) that improves meiosis resumption in vitro of porcine COCs, 2) cationic 18 kDa protein(s) are responsible for promotion of MⅡ stage.

참고 자료

없음

자료문의

제휴사는 별도로 자료문의를 받지 않고 있습니다.

판매자 정보

코리아스칼라는 정직과 신뢰를 기반으로 학술단체 발전에 도움을 드리고자 하는 기업입니다. 본 사는 본 사가 자체 개발한 솔루션을 통하여 보다 효율적인 업무 관리 뿐만 아니라, 학술지의 데이터베이스화, ARCHIVE를 돕습니다. 본 사의 One Stop Service를 통해 국제적인 학술단체로 함께 도약 할 수 있다고 믿습니다.

본 학술논문은 (주)코리아스칼라와 각 학회간에 저작권계약이 체결된 것으로 AgentSoft가 제공 하고 있습니다.
본 저작물을 불법적으로 이용시는 법적인 제재가 가해질 수 있습니다.

상세하단 배너
우수 콘텐츠 서비스 품질인증 획득
최근 본 자료더보기
Effect of Epididymal Fluid Fractionated by Chromatography on In Vitro Maturation of Porcine Follicular Oocytes