RE-10 : Effect of Di (2-ethylhexyl) phthalate on human endometrial stromal cells, on the repsect of oxidative stress

저작시기 2014.01 |등록일 2015.01.05 | 최종수정일 2015.04.23 파일확장자어도비 PDF (pdf) | 1페이지 | 가격 1,000원
다운로드
장바구니관심자료
상세신규 배너

* 본 문서는 배포용으로 복사 및 편집이 불가합니다.

서지정보

발행기관 : 대한산부인과학회 수록지정보 : 대한산부인과학회 학술발표논문집 / 100권
저자명 : ( Yeon Jean Cho ) , ( Seung Bin Park ) , ( Jong Woon Bae ) , ( Jung Woo Park ) , ( Hee Suk Lee ) , ( Myoung Seok Han )

없음

영어 초록

목적: Di-(2-ethylhexyl)-phthalate (DEHP), one of the most commonly used phthalate diesters, and is considered to be a reproductive and development toxicants in humans and animals. It may induce oxidative stress in other human cells. We aimed to see if DEHP induce oxidative stress in human endometrial stromal cell in vitro, which could be one of the background of explaining the pathogenesis endocrine-related diseases related with the endometrium. 방법: For endometrial stromal cell (ESC) cultures, endometrial samples were obtained from disease free premenopausal women. We treated a human endometrial stromal cell with DEHP and measured reactive oxygen species (ROS) generation using the dichlorofluorescein diacetate (DCF-DA) assay. Quantitative real time PCR (qPCR) for antioxidant enzymes, hormonal receptors and proinflammatory cytokines. Western blot analysis for nuclear protein was done. 결과: We showed that DEHP increases reactive oxygen species (ROS) and decreases antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPX), heme oxygenase (HO) and catalase (CAT). Increased ROS activates the MAPK/NF-κB signaling pathway and subsequently increased production of nitric oxide (NO). Increased production of proinflammatory cytokines such as IL-6 and TNF-a were noted. We also found that the mRNA and protein expression levels of ER-α were up-regulated in DEHP treated human endometrial stromal cells. In contrast, there was a decrease in ER-β (statistically significant) and PR expression (statistically non-signifcant). 결론: In conclusion, we found that DEHP induce oxidative stress in human endometrial stromal cells, with alterations of intracellular mechansism. It may contribute to endocrine-related disease such as endometriosis or adenomyosis.

참고 자료

없음
  • 구매평가(0)
  • 구매문의(0)
      최근 구매한 회원 학교정보 보기
      1. 최근 2주간 다운받은 회원수와 학교정보이며
         구매한 본인의 구매정보도 함께 표시됩니다.
      2. 매시 정각마다 업데이트 됩니다. (02:00 ~ 21:00)
      3. 구매자의 학교정보가 없는 경우 기타로 표시됩니다.
      4. 지식포인트 보유 시 지식포인트가 차감되며
         미보유 시 아이디당 1일 3회만 제공됩니다.
      상세하단 배너
      우수 콘텐츠 서비스 품질인증 획득
      최근 본 자료더보기
      상세우측 배너
      상세우측 배너
      RE-10 : Effect of Di (2-ethylhexyl) phthalate on human endometrial stromal cells, on the repsect of oxidative stress
      페이지
      만족도 조사

      페이지 사용에 불편하신 점이 있으십니까?

      의견 보내기