[독성]In vitro cytotoxic and genotoxic effects of diphenylarsinic acid,a degradation product of chemical warfare agents
- 최초 등록일
- 2006.03.30
- 최종 저작일
- 2005.08
- 28페이지/ MS 파워포인트
- 가격 3,000원
소개글
In vitro cytotoxic and genotoxic effects of diphenylarsinic acid,a degradation product of chemical warfare agents
목차
Abstract
Materials and methods
Cell culture
Cytotoxicity
Result
Discussion
본문내용
Abstract
Cultured human cells or animal cells에서의 GSH(glutathion)결핍에 대한 DPAs(V)의 cytotoxicity & genotoxicity의 관계 조사
세포분열의 Abnormal mechanism에 관련된 centrosome integrity와 microtubule organization에 미치는 DPAs(V)의 영향
DPAs(V)의 cytotoxicity에 대한 SH compounds의 영향
Materials and methods
Chemicals
Cell culture
Treatment of cells with the compounds
Determination of cell glutathione
Cytotoxicity
염색체와 mitotic index의 구조적, 수적변화
Immunofluorescence analysis of tubulins
Cell culture
. HepG2 cell
Human hepatocarcinoma
DMEM supplemented with 10% FBS
→ Grown in a monolayer
2. V79 cell
The lung fibroblasts of a chinese hamster(♂)
EMEM supplemented with 10% FBS
→ Grown in a monolayer
☞ Incubating in 5% CO2 in humidified air
Treatment of cells with the compounds
DPAs(V)
→ Dissolved in DMSO as a stock solution of 0.25M
→ Diluted with culture medium before use
DMSO control
→ Be prepared according to the final conc. of DMSO in each DPAs(V)-treated group
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