벼(동안) 식물조직배양 레포트

목차

1. Materials
2. Date
3. Method
4. Results
5. Discussion
6. References

본문내용

Seed sterilization
Dehusk mature healthy seeds surface-sterilize in 70% ethanol for 1 min, then in 70% Clorox for 30min with vigorous shaking, followed by five rinses in sterile water.

Preparation of calli
1. Place ten sterilized seeds per plate on the surface of N6D medium and seal the plate with surgical tape or wrap.
2. incubate the seeds on the medium at 28℃ for 3-4 weeks in dark.
3. calli are formed from the scutella after 3-4 weeks in culture.

Regeneration and Rooting
1. Transfer the growing calli to NR medium for shoot regeneration.
2. Seal plates with surgical tape or wrap.

참고 자료

그림으로 해설한 식물조직배양입문 / 성안당 / 박해준 / 12p, 52p, 141p, 144p
생물과학 제3판 / 아카데미서적 / 강신성 외 7명 / 305p
최신식물생리학 / 아카데미서적 / 권영명 외 14명 / 311p
강의자료 참조.