Hela Cell Culture 세포배양

소개글

Understand the basic principles and techniques of cell subculture using HeLa cell; remove medium from culture container, rinse with PBS, expose cells to trypsin, incubate, stop trypsin activity by adding FBS containing medium, dissociate cells, dilute and return to incubator.

목차

Purpose
Introduction
Material
Method
Result
Discussion

본문내용

Purpose
Understand the basic principles and techniques of cell subculture using HeLa cell; remove medium from culture container, rinse with PBS, expose cells to trypsin, incubate, stop trypsin activity by adding FBS containing medium, dissociate cells, dilute and return to incubator.

Introduction
A cell line is defined as a cell culture following its first passage. Cell lines may either be continuous (immortal) or finite (limited lifespan). Most human continuous cell lines in routine use are derived from cancers. There are increasing numbers of human cell lines derived from normal embryonic or adult tissues and cancers that have been immortalized with viral genes or other recombinant DNA (1).
HeLa cells are one of the cell lines that are “immortal”-they can grow indefinitely, be frozen for decades, Maryland, created the first immortal human cell line with a tissue sample taken from a young black woman with cervical cancer. Medical researchers these cells to learn the intricacies of how cells work and test theories about the causes and treatment of diseases, therefore quickly became invaluable to medical research (2).

참고 자료

(1) John R Masters, Glyn N Stacey. Changing medium and passaging cell lines. Nture protocols (2007)
(2) Sarah Zielinski. Henrietta Lacks` `Immortal` Cells. Smithsonian magazine (2010)