돼지 체외수정란 및 단위발생란의 미세관 형성 및 메틸화 양상

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 33권 / 1호
ㆍ저자명 : 박주희, 김호정, 이범기, 권대진, 황인선, 박춘근, 양부근, 정희태

목차

ABSTRACT 서론 재료 및 방법 난포란의 채취 및 성숙배양 체외수정 단위발생 체외배양 면역형광염색을 통한 핵형 및 Tubulin 관찰 Confocal Microscopy를 이용한 메틸화 검사 통계 처리 결과 체외수정란 및 단위발생란의 미세관 형태 체외수정란 및 단위발생란의 매틸화 양상 고찰 인용문헌

영어 초록

This study was performed to confirm the microtubule assemblies and methylation patterns of porcine IVF and parthenogenetic embryos. Cumulus-oocyte complexes were collected and matured in vitro for 42 hr. Oocytes were fertilized by prepared fresh sperm or activated parthenogenetically by exposure to electric stimulation and 6-dimethylaminopurine. Porcine IVF and parthenogenetic embryos were cultured in vitro for 6 days. Embryos were stained by immunofluorescence staining method to observe the dynamic of nucleus and microtubules in the first mitotic phase and the methylation patterns in different developmental stages. After then, samples were confirmed and analyzed through a laser-scanning confocal microscope. IVF embryos had a centrosome originated from sperms, which was shown a ɤ-tubulin spot. However, ɤ-tubulin spot was not observed in parthenogenetic embryos. A lower methylation level was observed in IVF embryos compared to parthenogenetic ones at the morula and blastocyst stages. In conclusion, it is considered that microtubule assemblies and genetic regulation mechanism differ between parthenogenetic and IVF embryos.

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