Campbell Biology 17- gene to protein text
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- 2011.11.23
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Campbell ppt
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Using genetic crosses
They determined that their mutants fell into three classes, each mutated in a different gene
Figure 7.2
Working with the mold Neurospora crassa, George Beadle and Edward Tatum had isolated mutants requiring arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metabolic pathway of arginine biosynthesis included the precursors ornithine and citrulline. Their most famous eperiment, shown here, tested both their one gene?one enzyme hypothesis and their postulated arginine pathway. In this eperiment, they grew their three classes of mutants under the four different conditions shown in the Results section below.
The wild-type strain required only the minimal medium for growth. The three classes of mutants had different growth requirements
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